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Visual and multiplex detection of nucleic acid sequence by multiple cross displacement amplification coupled with gold nanoparticle-based lateral flow biosensor
- Source :
- Sensors and Actuators B: Chemical. 241:1283-1293
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- Here, we reported on the establishment of a nearly instrument free, simple and rapid molecular technique, which incorporated multiple cross displacement amplification coupled with lateral flow biosensor (MCDA-LFB) for the visual, sensitive and specific detection of nucleic acid sequence. The MCDA-LFB assay was able to simultaneously detect and correctly differentiate multiple targets using a 40 min amplification reaction followed by a 2 min incubation of the products on the visualization biosensor. The biosensor was devised to detect three targets, a chromatography control, the MCDA target amplicon I and II, and the interpretation of test results is based on the appearance of red lines on the reaction pad. As a proof of concept, Shigella and Salmonella strains were detected by MCDA-LFB technique to demonstrate the availability of target analysis. The analytical sensitivity, specificity and practical application of MCDA-LFB assay were successfully evaluated in pure culture and blood samples, and the whole procedure, including specimen (blood sample) processing (35 min), isothermal reaction (40 min), and result reporting (5 min), was completed within 80 min. Therefore, the simplicity, rapidity and nearly apparatus-free platform of the MCDA-LFB assay make it practical for point-of-care testing, field detection, ‘on-site’ diagnosis and more.
- Subjects :
- 0301 basic medicine
Materials science
Chromatography
Metals and Alloys
Nucleic acid sequence
Analytical chemistry
Nanoparticle
Target analysis
Amplicon
Condensed Matter Physics
Displacement (vector)
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Field detection
03 medical and health sciences
030104 developmental biology
Materials Chemistry
Multiplex
Electrical and Electronic Engineering
Instrumentation
Biosensor
Subjects
Details
- ISSN :
- 09254005
- Volume :
- 241
- Database :
- OpenAIRE
- Journal :
- Sensors and Actuators B: Chemical
- Accession number :
- edsair.doi...........44d1746ab57255e603389dcd85f96072