A glycine-containing phosphorus-free lipoaminoacid from the gram-negative marine bacterium Cyclobacterium marinus WH

A phosphorus-free acidic lipid was isolated from the low-polarity fraction of extractable lipids of the gram-negative marine bacterium Cyclobacterium marinus WH . Using IR and 1 H NMR spectroscopy, mass spectrometry, chromatographic techniques, and chemical degradation experiment, the lipid was established to be N -[3- d -(13′-methyltetradecanoyloxy)-15-methylhexadecanoyl]glycine. It constituted about 5% of the total lipids. The same or at least closely related lipid was found to occur in gram-negative fresh-water bacteria belonging to the genera Arcocella and Flectobacillus.