Isozyme analysis of Kenyan phlebotomine sandflies (Diptera: Ppsychodidae) by isoelectric focusing (IEF) on pharmacia phast system™

Key Word Index-Phlebotominae, Phlebotomus, Sergentomyia. Abstract-The isoenzyme profiles of nine sandfiy species (Phlebotomus duboscqi, P. elgonensis, P. manini, P. pedifet; 5ergentomiya antennatus, S. bedfordi, S. gamhami, S. graingeri and S. schwetzJl from Kenya coUected from the field and reared in the Medical Vectors Research Programme of the ICIPE were determined by isoelectric focusing (IEF) on mini ultra-thin polyacrylamide gels supplied by Pharmacia. Three of the nine isoenzymes screened (MDH, G-6-PD, PGM, ME, lCD, GAPDH, 6-POG, HK and GPII, namely GP!, PGM and ME, gave banding panerns of taxonomic value. Cluster analysis of the simple matching coeffi· cients gave two distinct groupings corresponding to previously described Sergentomyia and Phlebotomus respectively. However, S. gamhami, previously grouped in the genus Sergenromyia, was found to be associ­ atad with the genus Phlebotomus.