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Characterization, Phylogeny, and Genome Analyses of Nonpathogenic Xanthomonas campestris Strains Isolated from Brassica Seeds
- Source :
- Phytopathology®. 110:981-988
- Publication Year :
- 2020
- Publisher :
- Scientific Societies, 2020.
-
Abstract
- Xanthomonads were detected by using the Xan-D(CCF) medium from the brassica seeds, and their pathogenicity was determined by plant inoculation tests. It was found that some seed lots were infested with Xanthomonas campestris pv. campestris, some with X. campestris pv. raphani, and some with nonpathogenic xanthomonads. The nonpathogenic xanthomonad strains were identified as X. campestris, and the multilocus sequence analysis showed that the nonpathogenic X. campestris strains were grouped together with pathogenic X. campestris, but not with nonpathogenic strains of X. arboricola. In addition, all isolated X. campestris pv. campestris and X. campestris pv. raphani strains were positive in the hrpF-PCR, but the nonpathogenic strains were negative. It was further found that nonpathogenic X. campestris strain nE1 does not contain the entire pathogenicity island (hrp gene cluster; type III secretion system) and all type III effector protein genes based on the whole genome sequence analyses. The nonpathogenic X. campestris strain nE1 could acquire the entire pathogenicity island from the endemic X. campestris pv. campestris and X. campestris pv. raphani strains by conjugation, but type III effector genes were not cotransferred. The studies showed that the nonpathogenic X. campestris strains indeed exist on the brassica seeds, but it could be differentiated by the PCR assays on the hrp and type III effector genes. Nevertheless, the nonpathogenic X. campestris strains cannot be ignored because they may be potential gene resources to increase genetic diversity in the endemic pathogenic X. campestris pv. campestris and X. campestris pv. raphani strains.
- Subjects :
- 0301 basic medicine
biology
Effector
Sequence analysis
030106 microbiology
Plant Science
biology.organism_classification
Pathogenicity island
Xanthomonas campestris
Type three secretion system
Microbiology
03 medical and health sciences
030104 developmental biology
Gene cluster
Bacteriology
Agronomy and Crop Science
Gene
Subjects
Details
- ISSN :
- 19437684 and 0031949X
- Volume :
- 110
- Database :
- OpenAIRE
- Journal :
- Phytopathology®
- Accession number :
- edsair.doi...........43a7e83da1576f6d0084f12b291bcf2f