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The nuclear lamina couples mechanical forces to cell fate in the preimplantation embryo via actin organization

Authors :
Robin M. Skory
Adam A. Moverley
Goli Ardestani
Yanina Alvarez
Ana Domingo-Muelas
Oz Pomp
Blake Hernandez
Piotr Tetlak
Stephanie Bissiere
Claudio D. Stern
Denny Sakkas
Nicolas Plachta
Source :
Nature Communications. 14
Publication Year :
2023
Publisher :
Springer Science and Business Media LLC, 2023.

Abstract

During preimplantation development, contractile forces generated at the apical cortex segregate cells into inner and outer positions of the embryo, establishing the inner cell mass (ICM) and trophectoderm. To which extent these forces influence ICM-trophectoderm fate remains unresolved. Here, we found that the nuclear lamina is coupled to the cortex via an F-actin meshwork in mouse and human embryos. Actomyosin contractility increases during development, upregulating Lamin-A levels, but upon internalization cells lose their apical cortex and downregulate Lamin-A. Low Lamin-A shifts the localization of actin nucleators from nucleus to cytoplasm increasing cytoplasmic F-actin abundance. This results in stabilization of Amot, Yap phosphorylation and acquisition of ICM over trophectoderm fate. By contrast, in outer cells, Lamin-A levels increase with contractility. This prevents Yap phosphorylation enabling Cdx2 to specify the trophectoderm. Thus, forces transmitted to the nuclear lamina control actin organization to differentially regulate the factors specifying lineage identity.

Details

ISSN :
20411723
Volume :
14
Database :
OpenAIRE
Journal :
Nature Communications
Accession number :
edsair.doi...........437ed692e9478ca85927c19bc69e0b0c
Full Text :
https://doi.org/10.1038/s41467-023-38770-5