Systematic dissection of the regulatory logic of transcriptional activation by TP53 binding sites

This deposition contains supplementary data files and laboratory notebooks of the manuscript 'Systematic dissection of the regulatory logic of transcriptional activation by TP53 binding sites'. In this study, thousands of barcoded TP53 reporters were systematically designed and probed in MCF7 and TP53-KO MCF7 cells upon stimulation with Nutlin-3a. Barcodes in the cDNA were quantified by high-throughput sequencing. Among others, the results demonstrate that TP53 drives transcription cooperatively from adjacent TP53 binding sites, and that the positioning of the TP53 binding sites is crucial for transcriptional activation. The files deposited here include: Laboratory notebooks (pdfs printedfrom labguru). This includes the generation of the plasmid pools, the library transfection protocols, and the western blotting procedure. Raw gel images etc. are available in this folder. Clustered barcode count files. These are the raw barcode counts that were extracted from the sequencing files and clustered using starcode. A reporter information sheet. This file includes a table containing all relevant reporter information and reporter sequences. A reporter activity sheet. This file includes the calculated reporter activities and can be used to recreate the figures presented in the manuscript. A supplementary table containing the primer sequences used in this study. &nbsp