Functional characterization, structural basis, and regio-selectivity control of a promiscuous flavonoid 7,4′-di-O-glycosyltransferase from Ziziphus jujuba var. spinosa

We report a promiscuous flavonoid 7,4′-di-O-glycosyltransferase (ZjOGT3), which shows high catalytic efficiency and regio-selectivity. And the regio-selectivity of ZjOGT3 was controlled by intrinsic reactivity and the enzyme microenvironment.