A Rapid High‐Performance Liquid Chromatographic Determination of Propylthiouracil

A rapid and sensitive high‐performance liquid chromatographic method was developed for the determination of propylthiouracil. The chromatographic system utilized a C8 column with the detector wavelength set at 276 nm. The mobile phase was composed of water and methanol in a 70:30 (v/v) ratio and was delivered at a flow rate of 0.5 mL/minute. The analytical run time was less than 7 min. The standard curves were linear over a range of 1–100 µg/mL with a correlation coefficient of 0.9958. The limit of quantification was assessed to be 15 ng and the limit of detection 4.5 ng. The RSD values for the intra‐ and inter‐day precision studies were 0.11–1.28% and 1.65%, respectively. The accuracy was ≤5% over the concentration range 1–100 µg/mL. The stability‐indicating nature of the assay method was determined by subjecting a stock solution of propylthiouracil to extremes of acidic and basic degradation. It was found that the method was specific for propylthiouracil. Hence, a simple, rapid, sensitive, stab...