Light controlled reversible Michael addition of cysteine: a new tool for dynamic site-specific labeling of proteins

Authors :: Soham Maity
Courtney Bingham
Wei Sheng
Nona Ehyaei
Debarshi Chakraborty
Setare Tahmasebi-Nick
Thomas E. Kimmel
Chrysoula Vasileiou
James H. Geiger
Babak Borhan
Source :: The Analyst. 148:1085-1092
Publication Year :: 2023
Publisher :: Royal Society of Chemistry (RSC), 2023.
Abstract: An engineered hCRBPII protein binds a coumarin-based fluorophore to yield a photoswitchable system via Michael/retro-Michael addition of a cysteine residue. Alternate UV/visible light irradiation accesses the two optical states.

Subjects :: Electrochemistry
Environmental Chemistry
Biochemistry
Spectroscopy
Analytical Chemistry

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