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Functional Interaction of STAT3 Transcription Factor with the Cell Cycle Inhibitor p21
- Source :
- Journal of Biological Chemistry. 275:18794-18800
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- Signal transducers and activators of transcription (STAT) factors are cytoplasmic proteins that induce gene activation in response to cytokine receptor stimulation. Following tyrosine phosphorylation, STAT proteins dimerize, translocate into the nucleus, and activate specific target genes. Activation is transient, and down-regulation of STAT signaling occurs within a few hours. In the present study, we show that the cyclin-dependent kinase inhibitor p21(WAF1/CIP1/SDI1) inhibits STAT3 transcriptional activation. Following leukemia inhibitory factor stimulation, p21(WAF1/CIP1/SDI1) was found to associate with STAT3 proteins in coimmunoprecipitation and pull down assays. In vivo, overexpression of p21(WAF1/CIP1/SDI1) reduced transcriptional activation by STAT3 proteins but did not modify DNA binding activity. Interestingly, pull down experiments showed that p21(WAF1/CIP1/SDI1) could interact with the CREB-binding coactivator protein, and inhibition of STAT3 activity by p21(WAF1/CIP1/SDI1) did not occur when CREB-binding protein was overexpressed. These results suggest a model by which p21(WAF1/CIP1/SDI1) functions as an inhibitor of STAT3 signaling and highlight a new activity for this cyclin-dependent kinase inhibitor.
- Subjects :
- biology
Tyrosine phosphorylation
Cell Biology
Biochemistry
DNA-binding protein
Molecular biology
Cell biology
chemistry.chemical_compound
chemistry
Coactivator
biology.protein
STAT protein
Protein inhibitor of activated STAT
biological phenomena, cell phenomena, and immunity
CREB-binding protein
STAT3
neoplasms
Molecular Biology
STAT6
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 275
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi...........401ebce2d9a301d1b710350f9528c9b3