Residues of sulfadimethoxine in blood and liver of cultured olive flounder Paralichthys olivaceus by oral administration

The residue levels of sulfadimethoxine (SDM) was studied after oral administration to cultured olive flounder, Paralichthys olivaceus at 20±1.0℃. The concentrations of SDM in the plasma and liver were determined by HPLC-UV detector after a single dosage of 400 ㎎/㎏ body weight. The average recoveries of SDM in spiked samples between 2~50 ppm were 92.24~93.62% for plasma and 88.34~91.90% for liver. Limit of detection for SDM was 0.05 ppm by using this method. Samples were taken at 1 h, 6 h, 12 h, 24 h, 48 h, 72 h, 168 h, 240 h, 336 h and 480 h post-dose. The peak plasma and liver concentrations of SDM, which attained at 1 h post-dose, was 402.64±59.66 ㎍/㎖ and 238.18±54 ㎍/g, respectively. Thereafter, it’s elimination from both tissues was considerably faster following process of time. Their concentrations of SDM were not measurable at 480 h post-dose. Based on this results, dosage and withdrawal times for SDM could be used when it is prescribed with SDM in olive flounder.