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Performance comparison of deep sequencing platforms for detecting HIV-1 variants in the pol gene
- Source :
- Journal of Medical Virology. 90:1486-1492
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- The present study compares the performances of an in-house sequencing protocol developed on MiSeq, the Sanger method, and the 454 GS-FLX for detecting and quantifying drug-resistant mutations (DRMs) in the human immunodeficiency virus polymerase gene (reverse transcriptase [RT] and protease [PR]). MiSeq sequencing identified all the resistance mutations detected by bulk sequencing (n = 84). Both the MiSeq and 454 GS-FLX platforms identified 67 DRMs in the RT and PR regions, but a further 25 DRMs were identified by only one or other of them. Pearson's analysis showed good concordance between the percentage of drug-resistant variants determined by MiSeq and 454 GS-FLX sequencing (ρ = .77, P < .0001). The MiSeq platform is as accurate as the 454 GS-FLX Roche system for determining RT and PR DRMs and could be used for monitoring human immunodeficiency virus type 1 drug resistance.
- Subjects :
- 0301 basic medicine
Sanger sequencing
animal structures
Pol genes
Human immunodeficiency virus (HIV)
Drug resistance
Biology
medicine.disease_cause
Virology
Deep sequencing
Reverse transcriptase
03 medical and health sciences
symbols.namesake
030104 developmental biology
Infectious Diseases
Performance comparison
medicine
symbols
Polymerase Gene
Subjects
Details
- ISSN :
- 01466615
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- Journal of Medical Virology
- Accession number :
- edsair.doi...........3f6093f9a6a111a805cc39009704a6a4