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Multiplex species-specific PCR identification of native and non-native oysters (Crassostrea) in Brazil: a useful tool for application in oyster culture and stock management

Authors :
Adam Rick Bessa da Silva
Claudia Helena Tagliaro
Mauro André Damasceno de Melo
Colin Robert Beasley
Source :
Aquaculture International. 21:1325-1332
Publication Year :
2013
Publisher :
Springer Science and Business Media LLC, 2013.

Abstract

In an effort to develop molecular tools for oyster identification, this study reveals the usefulness of a multiplex polymerase chain reaction (PCR) for the reliable, rapid and low-cost identification of the four oyster species found along the Brazilian coast: Crassostrea gasar, Crassostrea rhizophorae, Crassostrea gigas and Crassostrea sp. Canela originally found at Para, Brazil. In order to perform a simultaneous identification of these species, we used a set of five primers developed and adapted from the cytochrome oxidase subunit I (COI) and internal transcribed spacer 1 (ITS1) fragments, respectively. Amplification was successful in all four species and PCR products were visualized in agarose gel. A single reaction was capable of distinguishing between the species: C. gigas, with two fragments (236 bp for COI and 718 bp for ITS1); C. gasar, with one fragment (718 bp for ITS1); Crassostrea sp., with one fragment (621 bp for ITS1) and C. rhizophorae with two fragments (377 bp for COI and 718 bp for ITS1). This molecular approach provides a simple and rapid identification of the oyster species from the Brazilian coast, thus increasing the efficient and quality of oyster culture programs by reducing the risk of wrong species identification.

Details

ISSN :
1573143X and 09676120
Volume :
21
Database :
OpenAIRE
Journal :
Aquaculture International
Accession number :
edsair.doi...........3f4a7d7ddaba09e0f8e4bd0238f0461c
Full Text :
https://doi.org/10.1007/s10499-013-9635-8