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Multiplex species-specific PCR identification of native and non-native oysters (Crassostrea) in Brazil: a useful tool for application in oyster culture and stock management
- Source :
- Aquaculture International. 21:1325-1332
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- In an effort to develop molecular tools for oyster identification, this study reveals the usefulness of a multiplex polymerase chain reaction (PCR) for the reliable, rapid and low-cost identification of the four oyster species found along the Brazilian coast: Crassostrea gasar, Crassostrea rhizophorae, Crassostrea gigas and Crassostrea sp. Canela originally found at Para, Brazil. In order to perform a simultaneous identification of these species, we used a set of five primers developed and adapted from the cytochrome oxidase subunit I (COI) and internal transcribed spacer 1 (ITS1) fragments, respectively. Amplification was successful in all four species and PCR products were visualized in agarose gel. A single reaction was capable of distinguishing between the species: C. gigas, with two fragments (236 bp for COI and 718 bp for ITS1); C. gasar, with one fragment (718 bp for ITS1); Crassostrea sp., with one fragment (621 bp for ITS1) and C. rhizophorae with two fragments (377 bp for COI and 718 bp for ITS1). This molecular approach provides a simple and rapid identification of the oyster species from the Brazilian coast, thus increasing the efficient and quality of oyster culture programs by reducing the risk of wrong species identification.
- Subjects :
- Oyster
animal structures
biology
Ecology
fungi
Zoology
Aquatic Science
Mangrove oyster
biology.organism_classification
law.invention
law
biology.animal
parasitic diseases
Multiplex polymerase chain reaction
Crassostrea
Identification (biology)
Multiplex
Internal transcribed spacer
Agronomy and Crop Science
Polymerase chain reaction
Subjects
Details
- ISSN :
- 1573143X and 09676120
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Aquaculture International
- Accession number :
- edsair.doi...........3f4a7d7ddaba09e0f8e4bd0238f0461c
- Full Text :
- https://doi.org/10.1007/s10499-013-9635-8