Additional file 2: Figure S2. of A new tool for tuberculosis vaccine screening: Ex vivo Mycobacterial Growth Inhibition Assay indicates BCG-mediated protection in a murine model of tuberculosis

Reproducibility of ex vivo MGIA. A) 5 × 106 splenocytes from mice immunized with BCG Pasteur Aeras (grey circles) or given saline (open squares) were co-cultured with 100 CFU of BCG Pasteur Aeras in four separate experiments. The MGIA was carried out 6 weeks after immunization with the exception of Exp. 2 (5 weeks after immunization). B) Data pooled from A). Each data point represents one animal. Error bars represent the median +/- interquartile range. Statistical significance was tested using the unpaired t test function in GraphPad Prism. (PDF 105 kb)