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[Untitled]
- Source :
- Journal of Assisted Reproduction and Genetics. 18:165-170
- Publication Year :
- 2001
- Publisher :
- Springer Science and Business Media LLC, 2001.
-
Abstract
- Purpose: To evaluate the effects of follicular fluid and platelet-activating factor on sperm motion characteristics of cryopreserved oligospermic and normospermic samples. Methods: Sperm motion characteristics were evaluated prior to cryopreservation, immediately after thawing and following incubation in human tubal fluid, follicular fluid, or 1-μM platelet-activating factor cultures. Sixteen oligospermic samples and 20 normospermic samples were examined. Sperm motion characteristics were analyzed manually according to WHO criteria (1999) and also with an automated videomicrography system. Result(s): Incubation in follicular fluid increased overall motility and the percentage of sperm with fast progressive motility in normospermic but not oligospermic samples. Incubation with platelet-activating factor increased overall motility and the percentage of sperm showing nonprogressive motility in both oligospermic and normospermic samples. Conclusion(s): The stimulatory effects of culture in follicular fluid as seen in normospermic samples do not show a significant benefit in oligospermic cryopreserved samples. Platelet-activating factor and follicular fluid increase motility via different mechanisms. Incubation of oligospermic cryopreserved sperm with PAF increases the number of motile sperm, thereby enabling easier identification of viable sperm for intracytoplasmic sperm injection in samples with severe asthenozoospermia.
- Subjects :
- endocrine system
urogenital system
medicine.medical_treatment
Obstetrics and Gynecology
Motility
General Medicine
Biology
medicine.disease
Asthenozoospermia
Sperm
Follicular fluid
Cryopreservation
Intracytoplasmic sperm injection
Andrology
Reproductive Medicine
Oligospermia
Genetics
medicine
reproductive and urinary physiology
Genetics (clinical)
Sperm motility
Developmental Biology
Subjects
Details
- ISSN :
- 10580468
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Journal of Assisted Reproduction and Genetics
- Accession number :
- edsair.doi...........3d1d62ca85eda04c52952b3f073a16b5