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ChemInform Abstract: Concise Synthesis of Ciguatoxin ABC-Ring Fragments and Surface Plasmon Resonance Study of the Interaction of Their BSA Conjugates with Monoclonal Antibodies
- Source :
- ChemInform. 32
- Publication Year :
- 2010
- Publisher :
- Wiley, 2010.
-
Abstract
- Monoclonal antibodies (mAbs), 4H2 and 6H7, were prepared previously using a protein conjugate of a 1:1 epimeric mixture of the synthetic ABC-ring fragments of ciguatoxin (CTX), 3 and 4. Here, the interactions of these mAbs with the fragments of CTX and CTX3C, 3 and 5, were investigated by surface plasmon resonance (SPR) spectroscopy in an attempt to clarify an antigenic determinant. Compared with the previous synthesis, the fragment 3 possessing the 2S configuration was synthesized from tri-O-acetyl-D-glucal much more effectively. The mAb 4H2 was already known to show a dose-dependent binding to the bovine serum albumin (BSA) conjugate of 3, but not to that of 5. The present SPR study of 4H2 demonstrates that the A-ring side chain of 3 plays a decisive role as an epitope. Therefore, SPR can effectively replace the ELISA method for the analysis of mAbs.
Details
- ISSN :
- 15222667 and 09317597
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- ChemInform
- Accession number :
- edsair.doi...........3d0451ccb8a5db7c8051f33a4843b3cc