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Modulation of Charge Density of Cationic Conjugated Polyelectrolytes for Improving the FRET-Induced Sensory Signal with Enhanced On/Off Ratio
- Source :
- Macromolecular Chemistry and Physics. 217:459-466
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- Three types of cationic polyfluorenes with a different number of ionic groups are synthesized to modulate their solubility in water and the interactions with anionic oligonucleotide probes (DNA-C). Increasing the charge density improves the water-solubility of conjugated polyelectrolytes (CPEs) but decreases its optical antenna effect in the electrostatic sensory platform (CPE/DNA-C), resulting in low fluorescence resonance energy transfer (FRET) signal. With increasing charge density in CPE, the concentration of conjugated units in CPE/DNA-C decreases at [+]:[−] = 1:1, after which little driving force is expected to bring positive CPEs and negative DNA-C into close proximity for efficient FRET. CPF2 with 2 ionic groups (per repeat unit) and hydrophilic polyether side-chains exhibits good water-solubility and a higher FRET signal with an enhanced signal on/off ratio. In addition, the charge density in CPEs modulates the range of sensory responses by forming a tighter or weaker electrostatic CPE/DNA-C complex, which perturbs the probe-target binding interactions.
- Subjects :
- Aqueous solution
Polymers and Plastics
Chemistry
Organic Chemistry
Analytical chemistry
Cationic polymerization
Ionic bonding
Charge density
02 engineering and technology
Conjugated system
010402 general chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
01 natural sciences
Conjugated Polyelectrolytes
0104 chemical sciences
Förster resonance energy transfer
Polymer chemistry
Materials Chemistry
Biophysics
Physical and Theoretical Chemistry
Solubility
0210 nano-technology
Subjects
Details
- ISSN :
- 10221352
- Volume :
- 217
- Database :
- OpenAIRE
- Journal :
- Macromolecular Chemistry and Physics
- Accession number :
- edsair.doi...........3cda36d093f264face9a160934bbb0ba