Epithelial and Neutrophil Interactions and Coordinated Response toShigellain a Human Intestinal Enteroid-Neutrophil Coculture Model

Studies of mucosal immunity and microbial host cell interaction have traditionally relied on animal models andin vitrotissue culture using immortalized cancer cell lines, which yield nonphysiological and often unreliable results. Herein, we report the development and characterization of anex vivoenteroid-PMN coculture consisting of normal human intestinal epithelium and a mechanistic interrogation of PMN and epithelial cell interaction and function in the context ofShigellainfection.