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Preparation and quantification of the total phenolic products inCitrusfruit using solid-phase extraction coupled with high-performance liquid chromatography with diode array and UV detection
- Source :
- Journal of Separation Science. 39:3806-3817
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Citrus fruit is an important health-promoting food that is rich in dietary phenolic metabolites. Traditional Chinese medicines, such as Zhishi and Zhiqiao, come from young and immature fruits of Citrus cultivars. The preparation of diversified bioactive phenolic products and establishment of the corresponding quality control methodology are challenging and necessary. In the current study, four types of solid-phase extraction sorbents for the enrichment and clean-up of the phenolic matrix were evaluated. A solid-phase extraction column coated with Strata-X was finally used in the procedure. Twenty phenolic compounds were selected to evaluate the extraction performances of the sorbents using high-performance liquid chromatography analysis. Under the optimized conditions, good linearities were obtained with R2 more than 0.9996 for all analytes with LODs of 0.04-1.012 μg/g. Intra- and interday relative standard deviation values were less than 3%, and the recovery was equal to or higher than 90.02%. Compared to non-solid-phase extraction process, the content of total phenolic products was elevated 35.55-68.48% with solid-phase extraction. Finally, the developed and validated method was successfully applied to the discrimination of Zhishi samples from different species as well as Zhishi and Zhiqiao samples in different development stages.
- Subjects :
- Detection limit
Analyte
Chromatography
010405 organic chemistry
010401 analytical chemistry
Extraction (chemistry)
Filtration and Separation
01 natural sciences
High-performance liquid chromatography
0104 chemical sciences
Analytical Chemistry
Matrix (chemical analysis)
chemistry.chemical_compound
chemistry
Phenols
Solid phase extraction
Citrus fruit
Subjects
Details
- ISSN :
- 16159306
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Journal of Separation Science
- Accession number :
- edsair.doi...........38fc6bdf06178a0a78ce87b7344bc36d