PP-28 MORPHOKINETIC FACTORS AS EMBRYO QUALITY PREDICTORS

Method: We analyzed fertilization of 1692 oocytes previously microinjected and cultured in EMBRYOSCOPE from April to December 2011. Out of these 1692, 1269 underwent normal fertilization (75%), 48 were uni-pronucleated (2.8%) and 63 were tri-pronucleated (3.7%). The remaining 312 oocytes suffered degeneration or had no pronuclei. The EMBRYOSCOPE is a time-lapse incubator that allows us to view, like in a film, the embryo development from the moment of ICSI until transfer by taking pictures of the embryos every 20 minutes in seven different depth fields. Thanks to the EMBRYOVIEWER, we are able to know the time after ICSI of many events that we consider to be important for the embryo evaluation, such as pronuclei appearance and disappearance, cell cleavage, etc. In addition, it allows us to measure certain parameters, such as pronuclei area and different lengths. We also registered the number of good quality embryos in day +3, considering these to be the A and B category embryos, according to the ASEBIR scale. After the ovarian puncture the oocytes are injected and cultured in EMBRYOSCOPE with GLOBAL culture media (LifeGlobal, IVFOnline) until day 3. Statistical analysis was performed with G-STAT. Differences were considered statistically significant at P< 0.05.