Construction of recombinant adenoviral vector expressing genes of the conservative proteins M2 'ion channel' and nucleoprotein of influenza A virus

Influenza is a highly contagious disease and one of the most wide spreaded infection in the world. Influenza type A strains are of very great epidemiological significance. A high degree of genetic variability leads to constant modification in the antigenic structure of influenza virus. Therefore, current influenza vaccines require periodic updating of the composition of the strains. At present, it is important to develop a universal vaccine that could protect against different strains of influenza A virus and it is can be based on the conserved antigens of the virus. Recombinant adenoviral vectors expressing genes of conserved viral antigens may be used for a promising candidate vaccine against influenza A. Using a homologous recombination method, in this study we have developed a recombinant adenoviral serotype 5 vector that expresses the genes of the M2 ion channel and nucleoprotein (NP) of influenza A virus. The genes of the consensus sequences of the M2 protein and NP of human influenza A virus were inserted into the structure of the viral genome. The expression of the M2 and NP antigens using the recombinant adenovirus vector in a permissive line of eukaryotic cells was detected by Western blot assay. The high immunogenicity of the recombinant adenovirus vector obtained was demonstrated by intranasal immunization of laboratory mice.