Granulocyte Activity Is Enhanced by Culture Supernatants of Mononuclear Leukocytes Incubated with Tetrachlorodecaoxide

Granulocytes (polymorphonuclear leukocytes, PMNs) and monocytes/ macrophages are important in wound healing. Within the first few hours PMNs and monocytes begin to infiltrate sites of injury. The major function of PMNs is to kill microbes and phagocytose cellular debris so that healing can proceed [1]. Monocytes/macrophages invading the wound are also active in phagocytosis. In addition they release a number of cytokines including growth factors, interleukin-8 (IL-8), and tumor necrosis factor (TNF-α) needed for new tissue formation and shown to stimulate PMNs [1–3].