Enzymatic Mineralization of Silk Scaffolds

Authors :: Mamoni Dash
Peter Dubruel
Tom Gheysens
Pascal Van Der Voort
Sangram Keshari Samal
Jolien Dendooven
Heidi Declercq
David L. Kaplan
Ria Cornelissen
Source :: Macromolecular Bioscience. 14:991-1003
Publication Year :: 2014
Publisher :: Wiley, 2014.
Abstract: The present study focuses on the alkaline phosphatase (ALP) mediated formation of apatitic minerals on porous silk fibroin protein (SFP) scaffolds. Porous SFP scaffolds impregnated with different concentrations of ALP are homogeneously mineralized under physiological conditions. The mineral structure is apatite while the structures differ as a function of the ALP concentration. Cellular adhesion, proliferation, and colonization of osteogenic MC3T3 cells improve on the mineralized SFP scaffolds. These findings suggest a simple process to generate mineralized scaffolds that can be used to enhanced bone tissue engineering-related utility.