Back to Search
Start Over
Dictyopteris undulata extract induces apoptosis in human colon cancer cells
- Source :
- Biotechnology and Bioprocess Engineering. 19:419-425
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- The present study investigated the cytotoxic and apoptotic effects of an ethanol extract derived from the marine brown alga Dictyopteris undulata against human colon adenocarcinoma cells. The Dictyopteris undulata extract (DUE) showed cytotoxic activity against SW480 cells in a dose-dependent manner, with 50% inhibition of cell viability at a concentration of 40 μg/mL. DUE also induced programmed cell death in SW480 cells, as evidenced by apoptotic body formation, DNA fragmentation, an increase in the population of apoptotic sub-G1 phase cells, and mitochondrial membrane depolarization. Moreover, DUE significantly modulated the expression of apoptosisassociated proteins, resulting in a decrease in B cell lymphoma-2 expression and an increase in Bcl-2-associated X protein expression, as well as the activation of caspase-9 and caspase-3. Furthermore, DUE showed apoptotic cell death in two other colon cancer cell lines, SNU407 and HT29. These observations suggest that DUE may prove useful as a therapeutic agent for the attenuation of colon cancer.
- Subjects :
- Programmed cell death
education.field_of_study
Population
Biomedical Engineering
Bioengineering
Biology
Apoptotic body
Applied Microbiology and Biotechnology
Molecular biology
digestive system diseases
medicine.anatomical_structure
Apoptosis
Immunology
medicine
DNA fragmentation
Cytotoxic T cell
Viability assay
education
B cell
Biotechnology
Subjects
Details
- ISSN :
- 19763816 and 12268372
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioprocess Engineering
- Accession number :
- edsair.doi...........345ed7f9bbbad9636fccd77adce3acab