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A Simple Potentiometric Biosensor based on Carboxylesterase for the Analysis of Aspartame
- Source :
- Sains Malaysiana. 51:2913-2924
- Publication Year :
- 2022
- Publisher :
- Penerbit Universiti Kebangsaan Malaysia (UKM Press), 2022.
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Abstract
- A potentiometric aspartame biosensor was fabricated by simply depositing the carboxylesterase (CES)-bonded poly(n-butyl acrylate-n-acryloxysuccimide) [CES-poly(nBA-NAS)] microspheres on a Ag/AgCl screen-printed pH selective electrode. The pH transducer was made from non-plasticized polyacrylate membrane containing a hydrogen ionophore and lipophilic salt. The immobilized CES enzyme catalyzed the enzymatic hydrolysis of aspartame to L-aspartic acid (L-Asp), L-phenylalanine and methanol. Potentiometric determination of aspartame concentration was performed by quantifying the hydrogen ion concentration produced from L-Asp. The potentiometric determination of aspartame exhibited good selectivity with near Nernstian response. The sensitivity of the biosensor was closed to the Nernstian value, i.e., 50-52 mV decade-1 with a dynamic linear response range from 10-5 to 10-2 M and detection limit approaching 10-6 M. The aspartame biosensor demonstrated good repeatability and reproducibility with relative standard deviation (RSD) of 1.9% and 1.6%, respectively (n=3). The potentiometric aspartame biosensor was demonstrated to be reliable for determining aspartame content in sweetener samples and was comparable to the conventional high-performance liquid chromatography (HPLC) method for aspartame analysis.
- Subjects :
- Multidisciplinary
Subjects
Details
- ISSN :
- 27350118 and 01266039
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Sains Malaysiana
- Accession number :
- edsair.doi...........3457d772b0391846dac971ed4985dcb0