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SMN complexes of nucleus and cytoplasm: A proteomic study for SMA therapy

Authors :
Glenn E. Morris
Heidi R. Fuller
Source :
Translational Neuroscience. 1:261-267
Publication Year :
2010
Publisher :
Walter de Gruyter GmbH, 2010.

Abstract

Reduced levels of the survival of motor neurons protein (SMN), cause the inherited neuromuscular disorder, spinal muscular atrophy (SMA). The majority of therapeutic approaches to date have been focused on finding ways to increase expression of functional SMN protein, though stabilization of SMN protein may also be an important consideration. SMN interacts, directly or indirectly, stably or transiently, with a large number of other proteins, some of which contribute to SMN stability and may therefore be potential targets for SMA therapy. We recently characterized the nuclear SMN interactome using LC-MALDI-TOF/TOF analysis of anti-SMN pull-downs and identified myb-binding protein-1a (Mybbp1a) as a novel partner. In light of interest in cytoplasm-specific roles of the SMN complex, we have applied the same approach to characterise the cytoplasmic SMN interactome. We now show that SMN complexes from HeLa cytoplasmic extracts differ significantly from those found in nuclear extracts, with gemin5, importinbeta and annexin A2 easily detected only in the cytoplasmic extracts, whereas interaction of SMN with Mybbp1a appears to occur only in the nucleus. SMN is ubiquitinylated and we also found proteins of the ubiquitin-proteasome system associated with SMN in the cytoplasm.

Details

ISSN :
20816936 and 20813856
Volume :
1
Database :
OpenAIRE
Journal :
Translational Neuroscience
Accession number :
edsair.doi...........344ce36b1937069fe35b26c4c183177e
Full Text :
https://doi.org/10.2478/v10134-010-0027-6