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Electrospray ionization mass spectrometric method for the determination of cannabinoid precursors:N-acylethanolamine phospholipids (NAPEs)
- Source :
- Journal of Mass Spectrometry. 34:761-767
- Publication Year :
- 1999
- Publisher :
- Wiley, 1999.
-
Abstract
- N-Acylethanolamine phospholipids (NAPEs) serve as endogenous precursors of N-acylethanolamines (NAEs), e.g. N-arachidonoylethanolamine (anandamide) and N-palmitoylethanolamine that are endogenous ligands of cannabinoid receptors. Under physiological conditions, NAPE is found in very low concentrations in mammalian tissue (3-12 nmol g(-1)). However, pathophysiological conditions may increase the endogenous NAPE levels, which again may cause an increase in endocannabinoid concentrations. This paper presents a simple and selective method for the determination of NAPE standards using negative electrospray ionization mass spectrometry (ESI-MS). The procedure provides complete positioning of all acyl and alkenyl groups contained in each NAPE species. The calibration curve for standard NAPE was linear over the range 100 fmol-50 pmol (0.1-50 ng) per injection. The lower limit of detection (signal-to-noise ratio of 3) was 100 fmol, implying that this method is superior to previous methods for the determination of NAPE. These results suggest that this ESI-MS method can be used to identify and quantify NAPE species in mammalian tissues and provide information on the corresponding NAEs to be released from the endogenous NAPE pool.
- Subjects :
- Chromatography
Cannabinoid receptor
Nape
medicine.medical_treatment
Electrospray ionization
Mass spectrometry
Endocannabinoid system
chemistry.chemical_compound
medicine.anatomical_structure
chemistry
Biochemistry
N-Acylethanolamine
medicine
N-Acylphosphatidylethanolamine
Cannabinoid
Spectroscopy
Subjects
Details
- ISSN :
- 10969888 and 10765174
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Journal of Mass Spectrometry
- Accession number :
- edsair.doi...........3385c101ee04dc0f22d59bea5d19ed3b