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Damage to DNA indicated by an electrically heated DNA-modified carbon paste electrode
- Source :
- Journal of Electroanalytical Chemistry. 506:143-148
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- The behaviour of an electrically heated carbon paste electrode (CPE) with the surface modified by a calf thymus double stranded (ds) DNA adsorption layer was investigated in the medium of a copper(II) complex with 1,10-phenanthroline (phen) as the chemical nuclease. Damage to DNA is affected strongly by the electrode temperature within the range of 22–38°C as indicated by the square-wave voltammetric signals of the DNA redox marker Co(phen)33+ as well as the DNA guanine moiety. No significant effect of temperature on the binding constant was found at the titration of Cu(phen)22+ with dsDNA in solution. Thus, the formation of radical species and irreversible DNA damage are affected by DNA/CPE heating. The heated electrode can be applied advantageously to the real-time detection of traces of DNA-damaging agents.
Details
- ISSN :
- 15726657
- Volume :
- 506
- Database :
- OpenAIRE
- Journal :
- Journal of Electroanalytical Chemistry
- Accession number :
- edsair.doi...........321735bd29fe2b033c9753057d6ec50a