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Exo-exo synergy between Cel6A and Cel7A fromHypocrea jecorina: Role of carbohydrate binding module and the endo-lytic character of the enzymes
- Source :
- Biotechnology and Bioengineering. 114:1639-1647
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Synergy between cellulolytic enzymes is essential in both natural and industrial breakdown of biomass. In addition to synergy between endo- and exo-lytic enzymes, a lesser known but equally conspicuous synergy occurs among exo-acting, processive cellobiohydrolases (CBHs) such as Cel7A and Cel6A from Hypocrea jecorina. We studied this system using microcrystalline cellulose as substrate and found a degree of synergy between 1.3 and 2.2 depending on the experimental conditions. Synergy between enzyme variants without the carbohydrate binding module (CBM) and its linker was strongly reduced compared to the wild types. One plausible interpretation of this is that exo-exo synergy depends on the targeting role of the CBM. Many earlier works have proposed that exo-exo synergy was caused by an auxiliary endo-lytic activity of Cel6A. However, biochemical data from different assays suggested that the endo-lytic activity of both Cel6A and Cel7A were 103 -104 times lower than the common endoglucanase, Cel7B, from the same organism. Moreover, the endo-lytic activity of Cel7A was 2-3-fold higher than for Cel6A, and we suggest that endo-like activity of Cel6A cannot be the main cause for the observed synergy. Rather, we suggest the exo-exo synergy found here depends on different specificities of the enzymes possibly governed by their CBMs. Biotechnol. Bioeng. 2017;114: 1639-1647. © 2017 Wiley Periodicals, Inc.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
biology
fungi
food and beverages
Substrate (chemistry)
Bioengineering
Cellulase
biology.organism_classification
Applied Microbiology and Biotechnology
carbohydrates (lipids)
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Enzyme
chemistry
Lytic cycle
Biochemistry
Hypocrea
biology.protein
Carbohydrate-binding module
Cellulose
Linker
Biotechnology
Subjects
Details
- ISSN :
- 00063592
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioengineering
- Accession number :
- edsair.doi...........320082eafae5d6be614f07f840d15b8c