Decolorization of Remazol Brilliant Blue R using a novel acyltransferase-ISCO ( in situ chemical oxidation) coupled system

The feasibility of a novel perhydrolase-ISCO ( in situ chemical oxidation) coupled technology for decolorization process of dye effluent was investigated. All the tested synthetic dyes, Malachite green chloride, Indigo Carmine, Rhodamine B, Remazol Brilliant Blue R (RBBR) and Orange I, could be decolorized using the novel process technology. In the RBBR effluent model, the optimized parameters for decolorization process using free acyltransferase as biocatalyst were as follows: 4 U/mL acyltransferase dosage, 40:1 molar ratio of ethyl acetate to hydrogen peroxide, and 80 mg/L RBBR original concentration in 20 mmol/L disodium hydrogen phosphate-citrate buffer, pH5.0. Under the optimal parameters for RBBR decolorization process, the maximum decolorization rate reached 81.11% at 30 °C for 6 h. The decolorization rate could be further increased to 95.08% in 10 min when acyltransferase CLEAs (Crossed-Linked Enzyme Aggregates) was used as biocatalyst. In the continuous batch process for RBBR decolorization, acyltransferase E-CLEAs (Encapsulated CLEAs with polyvinyl alcohol) could be recycled at least 6 times.