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Extracellular Vesicles Derived from Lactobacillus plantarum Increase BDNF Expression in Cultured Hippocampal Neurons and Produce Antidepressant-like Effects in Mice
- Source :
- Experimental Neurobiology. 28:158-171
- Publication Year :
- 2019
- Publisher :
- The Korean Society for Brain and Neural Science, 2019.
-
Abstract
- Gut microbiota play a role in regulating mental disorders, but the mechanism by which gut microbiota regulate brain function remains unclear. Gram negative and positive gut bacteria release membrane-derived extracellular vesicles (EVs), which function in microbiota-host intercellular communication. In the present study, we investigated whether Lactobacillus plantarum derived EVs (L-EVs) could have a role in regulating neuronal function and stress-induced depressive-like behaviors. HT22 cells treated with the stress hormone glucocorticoid (GC; corticosterone) had reduced expression of Bdnf and Sirt1, whereas L-EV treatment reversed GC-induced decreased expression of Bdnf and Sirt1. The siRNA-mediated knockdown of Sirt1 in HT22 cells decreased Bdnf4, a splicing variant of Bdnf, and Creb expression, suggesting that Sirt1 plays a role in L-EV-induced increase of BDNF and CREB expression. Mice exposed to restraint for 2-h daily for 14 days (CRST) exhibited depressive-like behaviors, and these CRST-treated mice had reduced expression of Bdnf and Nt4/5 in the hippocampus. In contrast, L-EV injection prior to each restraint treatment blocked the reduced expression of Bdnf and Nt4/5, and stress-induced depressive-like behaviors. Furthermore, L-EV treatment in CRST-treated mice also rescued the reduced expression of Bdnf, and blocked stress-induced depressive-like behaviors. These results suggest that Lactobacillus derived EVs can change the expression of neurotropic factors in the hippocampus and afford antidepressant-like effects in mice with stress-induced depression.
- Subjects :
- 0301 basic medicine
Gene knockdown
medicine.medical_specialty
biology
Chemistry
Hippocampus
Hippocampal formation
CREB
biology.organism_classification
03 medical and health sciences
Cellular and Molecular Neuroscience
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
Endocrinology
Corticosterone
Internal medicine
Lactobacillus
medicine
biology.protein
Neurology (clinical)
030217 neurology & neurosurgery
Glucocorticoid
Intracellular
medicine.drug
Subjects
Details
- ISSN :
- 20938144 and 12262560
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Experimental Neurobiology
- Accession number :
- edsair.doi...........313f494dd6654f632aab31f5fb6e4544