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Danlou Tablet Improves Chronic Intermittent Hypoxia-Induced Dyslipidemia and Arteriosclerosis by HIF-1α-Angptl4 mRNA Signaling Pathway
- Source :
- Chinese Journal of Integrative Medicine. 28:509-517
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- To detect whether Danlou Tablet (丹蒌片, DLT) regulates the hypoxia-induced factor (HIF)-1 α-angiopoietin-like 4 (Angptl4) mRNA signaling pathway and explore the role of DLT in treating chronic intermittent hypoxia (CIH)-induced dyslipidemia and arteriosclerosis. The mature adipocytes were obtained from 3T3-L1 cell culturation and allocated into 8 groups including control groups (Groups 1 and 5, 0.1 mL of cell culture grade water); DLT groups (Groups 2 and 6, 0.1 mL of 1,000 µg/mL DLT submicron powder solution); dimethyloxalylglycine (DMOG) groups (Groups 3 and 7, DMOG and 0.1 mL of cell culture grade water); DMOG plus DLT groups (Groups 4 and 8, DMOG and 0.1 mL of 1,000 µg/mL DLT submicron powder solution). Groups 1–4 used mature adipocytes and groups 5–8 used HIF-1 α-siRNA lentivirus-transfected mature adipocytes. After 24-h treatment, real-time polymerase chain reaction and Western blot were employed to determine the mRNA and protein expression levels of HIF-1 α and Angptl4. In animal experiments, the CIH model in ApoE−/− mice was established. Sixteen mice were complete randomly divided into 4 groups including sham group, CIH model group [intermittent hypoxia and normal saline (2 mL/time) gavage once a day]; Angptl4 Ab group [intermittent hypoxia and Angptl4 antibody (30 mg/kg) intraperitoneally injected every week]; DLT group [intermittent hypoxia and DLT (250 mg/kg) once a day], 4 mice in each group. After 4-week treatment, enzyme linked immunosorbent assay was used to detect the expression levels of serum total cholesterol (TC) and triglyceride (TG). Hematoxylin-eosin and CD68 staining were used to observe the morphological properties of arterial plaques. Angptl4 expression was dependent on HIF-1 α, with a reduction in mRNA expression and no response in protein level to DMOG or DLT treatment in relation to siHIF-1 α -transfected cells. DLT inhibited HIF-1 α and Angptl4 mRNA expression (P
- Subjects :
- medicine.medical_specialty
0211 other engineering and technologies
02 engineering and technology
030226 pharmacology & pharmacy
Lesion
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
ANGPTL4
Internal medicine
021105 building & construction
medicine
Pharmacology (medical)
medicine.diagnostic_test
Triglyceride
Chemistry
Intermittent hypoxia
General Medicine
Arteriosclerosis
medicine.disease
Endocrinology
Complementary and alternative medicine
Cell culture
medicine.symptom
Dyslipidemia
Subjects
Details
- ISSN :
- 19930402 and 16720415
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Integrative Medicine
- Accession number :
- edsair.doi...........3096e5e6c4a102b191c6856445a81b99