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Interleukin-21 Induces Short-Lived Effector CD8 + T Cells but Does Not Inhibit Their Exhaustion after Mycobacterium bovis BCG Infection in Mice
- Source :
- Infection and Immunity. 86
- Publication Year :
- 2018
- Publisher :
- American Society for Microbiology, 2018.
-
Abstract
- Interleukin 21 (IL-21) is a pleiotropic common cytokine receptor γ chain cytokine that promotes the effector functions of NK cells and CD8+ T cells and inhibits CD8+ T cell exhaustion during chronic infection. We found that the absolute number of short-lived effector CD8+ T cells (SLECs) (KLRG1high CD127low) decreased significantly in IL-21 receptor-deficient (IL-21R-/-) mice during Mycobacterium bovis bacillus Calmette-Guerin (BCG) infection. Early effector CD8+ T cells (EECs) (KLRG1low CD127low) were normally generated in IL-21R-/- mice after infection. Exhausted CD8+ T cells (PD-1high KLRG1low) were also normally generated in IL-21R-/- mice after infection. Mixed bone marrow (BM) chimera and transfer experiments showed that IL-21R on CD8+ T cells was essential for the proliferation of EECs, allowing them to differentiate into SLECs after BCG infection. On the other hand, the number of SLECs increased significantly after infection with recombinant BCG (rBCG) that secreted an antigen 85B (Ag85B)-IL-21 fusion protein (rBCG-Ag85B-IL-21), but the number of exhausted CD8+ T cells did not change after rBCG-Ag85B-IL-21 infection. These results suggest that IL-21 signaling drives the differentiation of SLECs from EECs but does not inhibit the exhaustion of CD8+ T cells following BCG infection in mice.
- Subjects :
- 0301 basic medicine
medicine.medical_treatment
T cell
Immunology
Biology
Microbiology
Molecular biology
03 medical and health sciences
Interleukin 21
Chronic infection
030104 developmental biology
0302 clinical medicine
Infectious Diseases
Cytokine
medicine.anatomical_structure
Antigen
medicine
Cytotoxic T cell
Parasitology
Cytokine receptor
CD8
030215 immunology
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi...........308835d78ee6af94362f93d23567de1a