New Insights into Light-Induced Deactivation of Active Rhodopsin by SVD and Global Analysis of Time-Resolved UV/Vis- and FTIR-Data

Time-resolved Fourier transform infrared (FTIR) and UV/Vis difference spectra of light induced deactivation of the photoreceptor rhodopsin were simultaneously recorded on the same sample. The data were analyzed by a special designed combination of singular value decomposition and Global analysis to verify our recently published model of receptor deactivation. This mathematical approach enables us to obtain the pure difference spectra of the conversions between the species involved. We found two different species, which could be identified as key intermediates of the model. The new data allow deeper insights into the structural changes that come along with the formation of the deactivated state. By means of this method we can now separate and assign even strongly overlapping and highly coupled bands of the infrared difference spectra.