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[Untitled]
- Source :
- Journal of Biomolecular NMR. 9:313-316
- Publication Year :
- 1997
- Publisher :
- Springer Science and Business Media LLC, 1997.
-
Abstract
- A new method to selectively detect the ring resonances of the aromatic residues in 15N-labelled proteins is presented. The experiment consists of a 2D 1H TOCSY sequence withremoval of the amide signals via 15N-filtering. Experiments are acquired in the absence andpresence of water inversion; combining the two spectra allows selective observation of thetyrosine ring resonances and enables the identification of their delta andepsilon ring protons. The experiment is demonstrated on a 15N-labelled sample of Photoactive Yellow Protein and isshown to give good selectivity for tyrosine ring resonances under a wide range oftemperatures and pH values.
Details
- ISSN :
- 09252738
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of Biomolecular NMR
- Accession number :
- edsair.doi...........304a36f9ae30eb91d9ee059a3bb115ad