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Functional characterization and osmoregulatory role of the Na+-K+-2Cl−cotransporter in the gill of sea lamprey (Petromyzon marinus), a basal vertebrate
- Source :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 318:R17-R29
- Publication Year :
- 2020
- Publisher :
- American Physiological Society, 2020.
-
Abstract
- The present study provides molecular and functional characterization of Na+-K+-2Cl−cotransporter (NKCC1/Slc12a2) in the gills of sea lamprey ( Petromyzon marinus), the most basal extant vertebrate with an osmoregulatory strategy. We report the full-length peptide sequence for the lamprey Na-K-Cl cotransporter 1 (NKCC1), which we show groups strongly with and occupies a basal position among other vertebrate NKCC1 sequences. In postmetamorphic juvenile lamprey, nkcc1 mRNA was present in many tissues but was fivefold higher in the gill than any other examined tissue, and NKCC1 protein was only detected in the gill. Gill mRNA and protein abundances of NKCC1 and Na+-K+-ATPase (NKA/Atp1a1) were significantly upregulated (20- to 200-fold) during late metamorphosis in fresh water, coinciding with the development of salinity tolerance, and were upregulated an additional twofold after acclimation to seawater (SW). Immunohistochemistry revealed that NKCC1 in the gill is found in filamental ionocytes coexpressing NKA, which develop during metamorphosis in preparation for SW entry. Lamprey treated with bumetanide, a widely used pharmacological inhibitor of NKCC1, exhibited higher plasma Cl−and osmolality as well as reduced muscle water content after 24 h in SW; there were no effects of bumetanide in freshwater-acclimated lamprey. This work provides the first functional characterization of NKCC1 as a mechanism for branchial salt secretion in lampreys, providing evidence that this mode of Cl−secretion has been present among vertebrates for ~550 million years.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Gill
biology
Physiology
Lamprey
media_common.quotation_subject
Vertebrate
biology.organism_classification
010603 evolutionary biology
01 natural sciences
Cell biology
03 medical and health sciences
Basal (phylogenetics)
030104 developmental biology
Petromyzon
Physiology (medical)
biology.animal
Osmoregulation
Metamorphosis
Cotransporter
media_common
Subjects
Details
- ISSN :
- 15221490 and 03636119
- Volume :
- 318
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Regulatory, Integrative and Comparative Physiology
- Accession number :
- edsair.doi...........3021fb9f152b4708dcb3549c91f8b6d9