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Metrological support for DNA sequencing

Authors :
A. V. Mardanov
N. V. Ravin
S. A. Kononogov
Yu. A. Kudeyarov
S. S. Golubev
P. Yu. Nikolaeva
Konstantin G. Skryabin
Source :
Measurement Techniques. 55:345-350
Publication Year :
2012
Publisher :
Springer Science and Business Media LLC, 2012.

Abstract

We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.

Details

ISSN :
15738906 and 05431972
Volume :
55
Database :
OpenAIRE
Journal :
Measurement Techniques
Accession number :
edsair.doi...........2fa039ad77379dbcb1a42dbabad4d621