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Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants
- Source :
- Hereditas (Beijing). 31:663-667
- Publication Year :
- 2009
- Publisher :
- China Science Publishing & Media Ltd., 2009.
-
Abstract
- The AGPL1 (ADP-glucose pyrophosphorylase large subunit 1) promoter from watermelon (Citrullus vulgaris S.) has proved to exhibit fruit-specific expression patterns in tomato (Lycopersicon esculentum L.). A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.
Details
- ISSN :
- 02539772
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Hereditas (Beijing)
- Accession number :
- edsair.doi...........2f96dd160acc5ad3c46d3d577788d853