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Development and Validation of Uv-Spectrophotometric Method for the Determination of Atorvastatin Calcium Using Sodium Citrate as Hydrotropic Agent
- Source :
- Pharmaceutical Chemistry Journal. 54:422-429
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Simple, rapid, precise, accurate and specific UV-spectrophotometric method has been developed for atorvastatin calcium determination in bulk and in tablets dosage form. The method was based on using sodium citrate 0.01M as hydrotropic agent. Measurements of the UV absorbance were performed at 241 nm, and the developed method was validated as per ICH guidelines. The method obeyed Beer’s law (R2 = 0.999) in a concentration range of 2 – 20 μg/mL. The limits of detection (LOD) and quantification (LOQ) were 0.64 and 1.9 μg/mL, respectively. At all levels of precision, RSD% values were below 2%. Accuracy of the proposed method was ascertained by standard addition method and the percentage recovery (n = 3) was within 100 – 100.43%. The method was applied to analysis of three atorvastatin tablet brands each claimed to contain 20 mg atorvastatin, and the percentage drug content was found to be 101.42 ± 1.56%, 99.04 ± 0.33%, and 97.71 ± 0.98%.
- Subjects :
- Pharmacology
Detection limit
Chromatography
010405 organic chemistry
Chemistry
Atorvastatin
Uv absorbance
01 natural sciences
Dosage form
0104 chemical sciences
Drug content
010404 medicinal & biomolecular chemistry
chemistry.chemical_compound
Atorvastatin calcium
Standard addition
Drug Discovery
Sodium citrate
medicine
medicine.drug
Subjects
Details
- ISSN :
- 15739031 and 0091150X
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Pharmaceutical Chemistry Journal
- Accession number :
- edsair.doi...........2f96b70f5dd721c4741b42235215ae65