AB0183 Methotrexate induced apoptosis in synoviocyte of rheumatoid arthritis

Background Methotrexate (MTX) was the corner stone treatment of rheumatoid arhtirits. So for long time, MTX was used in rheumatoid arhtirits treatment with other Immunosuppressive drug. There were many hypothesis in mechanism of MTX on RA treatments, but accurate mechanism of MTX was not known Objectives To investigate the apoptotic mechanism of MTX in synoviocyte of rhuematoid arhtirits Methods Synovial cells were cultured from synovial fluid of Rhuematoid arhtirits (RA) patients and Osteoarhtirtis (OA) patients. In each group, the subculture was conducted for 5-6 passages and synovioctes was treated with 300μM 500μM MTX directly. In 400μM The cell viability was observed and apoptosis of synoviocyte was measured by flow cytometry Results MTX treated synovial cell death was higher RA than OA in 300μM 500μM MTX, In 400μM MTX, high Sub-G1 pick was observed and continued to 24 hours in RA synoviocytes Conclusions Apoptosis was the one of MTX mechasnism in RA treatment and RA synoviocytes apoptosis may be one of tool for RA treatment in the future References Determinants of Red Blood Cell Methotrexate Polyglutamate Concentrations in Rheumatoid Arthritis Patients Receiving Long-Term Methotrexate Treatment Lisa K. Stamp, John L. O’Donnell, Peter T. Chapman, Mei Zhang, Christopher Frampton, Jill James, Murray L. Barclay. Arthritis & Rheumatism Vol. 60, No. 8, August 2009, pp 2248–2256. Disclosure of Interest None Declared