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Chromatographic properties of the cadmium induced SOD isoform in Rhizopogon roseolus
- Source :
- Acta Physiologiae Plantarum. 20:229-233
- Publication Year :
- 1998
- Publisher :
- Springer Science and Business Media LLC, 1998.
-
Abstract
- Exposure of Rhizopogon roseolus mycelia to 15 mmol·dm−3 cadmium for 24 h induces a different pattern of Mn-SOD on polyacrylamide gels, probably being a changed form of an originally existing one. The presence of cadmium affects the chromatographic properties of this enzyme and its mobility through the acrylamide gel. This new isoform was purified using DEAE Trisacryl chromatography. Cadmium induced isoform adsorbed stronger to the ligands and was eluated with a Tris-HCl buffer containing 0.1 mol·dm−3 NaCl. SOD from control samples (not treated with cadmium) was eluated with the same buffer without NaCl.
- Subjects :
- inorganic chemicals
Gene isoform
chemistry.chemical_classification
Cadmium
Chromatography
biology
Physiology
Polyacrylamide
chemistry.chemical_element
Plant Science
biology.organism_classification
Superoxide dismutase
chemistry.chemical_compound
Enzyme
chemistry
Rhizopogon roseolus
biology.protein
Agronomy and Crop Science
Polyacrylamide gel electrophoresis
Mycelium
Subjects
Details
- ISSN :
- 18611664 and 01375881
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Acta Physiologiae Plantarum
- Accession number :
- edsair.doi...........2e4253af0fc041912581f85a2a995018