Evaluating Osteogenic Differentiation of Mesenchymal Stem Cells on Poly(caprolactone) Electrospun Scaffolds by Image Processing Techniques

Tissue engineering needs versatile methods as a mean to monitor morphological and functional success of the constructs such as scaffolds and implants. In this study, electrospun scaffolds based on poly(caprolactone) (PCL) were fabricated by electrospinning method and seeded by mesenchymal stem cells (MSCs) to investigate the morphological effects on osteogenic differentiation. Prior to cell seeding, substrates were modified and functionalized by increasing porosity via removal of sacrificial component and covalently grafting/adsorption of collagen type 1 (Col I) and nanohydroxy apatite particles (nHA). Images of the alizarin red stained substrate on day 21 of cell culture were obtained and by image processing techniques, extend of mineralization was evaluated. According to the results, mineral compound was more secreted from cells on the scaffolds with higher porosity and covalently functionalized surface.