The Biochemical investigation and analysis to determine a potent biomarker for HCC and HCV infections

The communicable Hepatitis C Virus (HCV) infection causes global health problems and deaths due to transmission by blood and body fluids and general symptoms include loss of appetite, fatigue, fever, abdominal pain, swelling, jaundice, and dark urine.This study contains a detailed study about the potent biochemical biomarkers for the diagnosis and detection of HCV infection. It includes the biochemical profiling relating to the HCV infection among both the genders of the human population is included in this paper.96 (5ml) EDTA blood samples of suspected HCV infected patients were collected from the different hospitals in Dehradun, Uttarakhand, India. The RNA extraction was performedwith the help of QIAamp Viral RNA mini kit (Cat. no. 52904). The HCV RNA quantification was achieved using the machine named Rotor Gene Q5 Plex Real-Time PCR and master mix was prepared by the Artus Amplification Kit from Qiagen.Out of 96 samples collected for HCV RNA biochemical profiling, 58 cases i.e., were found with the heavy load of virus and the study had 38 cases in which target virus was not detected. The biochemical investigation was performed on 96 cases with a high viral load of more than 34 IU/ml (≥34 IU/ml). The cases belonging to age 21-40 and 41-60 years were found to be most prominent among 35 and 37 cases respectively due to the repeated exposure to infected blood and the age group of 0-20 years was found to be least susceptible due to the protective antibody titer against HCV due to the vaccination in early age.