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Derivation of trophoblast stem cells unveils unrestrained potential of mouse ESCs and epiblast

Authors :
Debabrata Jana
Purnima Sailasree
Priya Singh
Mansi Srivastava
Vijay V Vishnu
Hanuman T Kale
Jyothi Lakshmi
Gunda Srinivas
Divya Tej Sowpati
P Chandra Shekar
Publication Year :
2023
Publisher :
Cold Spring Harbor Laboratory, 2023.

Abstract

SUMMARYmESCs and epiblast are considered to follow strict lineage adherence and lack the potential to contribute to trophoectoderm. Here, we report the derivation of trophoblast stem cells (ESTS) from the mESCs. The single-cell transcriptome and molecular characterization of ESTS show similarity with TSCs. They efficiently integrate into the TE compartment of the blastocyst and contribute to the placenta during development. We discovered GSK3β as a critical regulator of the TE fate of ESCs. It plays a vital stage-specific role during ESTS derivation. We further show β-CATENIN and an intron-I regulatory element ofCdx2are essential for the TE fate of ESCs. We further show that the mouse epiblast can readily differentiate into TE lineage. In contrast to the paradigm of the restricted potential of pluripotent ESCs and epiblast, our data shows that murine ESCs and epiblast have the unrestrained developmental potential for extraembryonic lineages.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........2d8d09eafcf96583ffd27b9d08c5d66a
Full Text :
https://doi.org/10.1101/2023.04.19.537518