New aliphatic and aromatic dialdehyde bridged turn-on probes for hypochlorite detection in biological samples based on bis(fluorescein)

The design, synthesis and photo-physical properties of two novel dialdehyde bridged turn-on fluorescent probes with dual C N moiety based on bis(fluorescein) for OCl− detection with high selectivity and sensitivity were reported. The well-defined linear plots between 5.0 μM and 500.0 μM of OCl− make the probes especially suitable for the quantification of OCl−. The ring-opening progress was investigated both experimentally and computationally. Fluorescent imaging for OCl− in living MG-63 cells have also been successfully performed.