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A Rapid Chemical Method for Detecting PPLO Contamination of Tissue Cell Cultures

Authors :
Robert T. Schimke
Michael F. Barile
Source :
Experimental Biology and Medicine. 114:676-679
Publication Year :
1963
Publisher :
SAGE Publications, 1963.

Abstract

SummaryA rapid, simple and routine chemical method for detection of PPLO contamination in cell cultures is reported. The method is based on the presence of arginine deiminase activity in all tissue culture PPLO strains, but absence from all mammalian tissue and PPLO-free cell cultures. In a total of 73 cell cultures examined, the detection of PPLO determined by presence of arginine deiminase activity agreed entirely with the most sensitive cultural procedure which requires 14 days.

Details

ISSN :
15353699 and 15353702
Volume :
114
Database :
OpenAIRE
Journal :
Experimental Biology and Medicine
Accession number :
edsair.doi...........2bc0ae1204ce315234ffd1096fdc28fe