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Characterization of the Promoter of Rice Plastid psaA-psaB-rps14 Operon and the DNA-Specific Binding Proteins
- Source :
- Plant and Cell Physiology.
- Publication Year :
- 1993
- Publisher :
- Oxford University Press (OUP), 1993.
-
Abstract
- Transcriptional analysis of the promoter of rice psaA-psaB-rps14 operon was performed with Bal 31 deleted mutants in spinach chloroplast extracts. Two functional promoters in this operon (denoted as "-175" and "-129" promoters) were revealed to be utilized by the spinach transcription machinery; however only the "-175" promoter is utilized in vivo. The existence of psaA promoter-specific binding factors in the chloroplast extracts was indicated by competitive gel retardation assay and exonuclease III protection analysis. After ammonium sulfate fractionation of the extracts followed by heparin-agarose column chromatography, the sequence-specific binding factors were characterized to be 66-kDa and 31-kDa polypeptides. Southwestern analysis demonstrated that the two protein factors interact independently with the psaA promoter-containing DNA fragment.
Details
- ISSN :
- 14719053
- Database :
- OpenAIRE
- Journal :
- Plant and Cell Physiology
- Accession number :
- edsair.doi...........2b2b4dd1f5ba2a0d38f624f6ccc53e05