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Manual MGIT™ system for the detection of Mycobacterium tuberculosis: insights from a high TB burden setting
- Source :
- The International Journal of Tuberculosis and Lung Disease. 20:605-610
- Publication Year :
- 2016
- Publisher :
- International Union Against Tuberculosis and Lung Disease, 2016.
-
Abstract
- Objective To evaluate the diagnostic performance of manual MGIT™ (MMGIT) compared to the gold standard, Lowenstein-Jensen (LJ), in the diagnosis of pulmonary tuberculosis (TB) in a high-burden setting. Methods Individuals with suspected TB enrolled in parallel diagnostic trials during 2007-2011 were included. Two samples were obtained from each patient and inoculated into MMGIT and LJ medium. Diagnostic tests were performed, and the incremental yield of a second test and time to detection (TTD) were calculated. Analyses were performed per patient and per sample. Gold standard was based on LJ culture. Results In the per patient and per sample analysis, we evaluated 1436 patients and 4142 samples. The sensitivity and specificity for smear and MMGIT per sample were respectively 89.9%/92.2% and 97.1%/98.9%. Contamination was observed in 1.4% of samples on MMGIT. The mean TTD (days) was 11.8 for MMGIT and 22.9 for LJ. The sensitivity and specificity for smear and MMGIT per patient were respectively 89.9% and 92.2% and 97.1% and 98.3%. A second MMGIT culture had an incremental yield of 1.6%. Conclusions MMGIT has high sensitivity and specificity, regardless of smear result, with a 50% reduction in TTD compared to LJ. These features make MMGIT an acceptable TB diagnostic method for use in resource-limited settings.
- Subjects :
- Pulmonary and Respiratory Medicine
medicine.medical_specialty
Time to detection
Diagnostic methods
Tuberculosis
biology
business.industry
Diagnostic test
Gold standard (test)
biology.organism_classification
medicine.disease
Mycobacterium tuberculosis
03 medical and health sciences
0302 clinical medicine
Infectious Diseases
Pulmonary tuberculosis
030220 oncology & carcinogenesis
Predictive value of tests
Internal medicine
medicine
030211 gastroenterology & hepatology
business
Subjects
Details
- ISSN :
- 18157920 and 10273719
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- The International Journal of Tuberculosis and Lung Disease
- Accession number :
- edsair.doi...........2b0d538740b9c9bc47c92f2ec2cf2cf4