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Manual MGIT™ system for the detection of Mycobacterium tuberculosis: insights from a high TB burden setting

Authors :
Eduardo Gotuzzo
Julio Huapaya
Carlos Zamudio
J Giraldo
I Novoa
G A Valle
R Quispe
Carlos Seas
Tatiana Cáceres
Source :
The International Journal of Tuberculosis and Lung Disease. 20:605-610
Publication Year :
2016
Publisher :
International Union Against Tuberculosis and Lung Disease, 2016.

Abstract

Objective To evaluate the diagnostic performance of manual MGIT™ (MMGIT) compared to the gold standard, Lowenstein-Jensen (LJ), in the diagnosis of pulmonary tuberculosis (TB) in a high-burden setting. Methods Individuals with suspected TB enrolled in parallel diagnostic trials during 2007-2011 were included. Two samples were obtained from each patient and inoculated into MMGIT and LJ medium. Diagnostic tests were performed, and the incremental yield of a second test and time to detection (TTD) were calculated. Analyses were performed per patient and per sample. Gold standard was based on LJ culture. Results In the per patient and per sample analysis, we evaluated 1436 patients and 4142 samples. The sensitivity and specificity for smear and MMGIT per sample were respectively 89.9%/92.2% and 97.1%/98.9%. Contamination was observed in 1.4% of samples on MMGIT. The mean TTD (days) was 11.8 for MMGIT and 22.9 for LJ. The sensitivity and specificity for smear and MMGIT per patient were respectively 89.9% and 92.2% and 97.1% and 98.3%. A second MMGIT culture had an incremental yield of 1.6%. Conclusions MMGIT has high sensitivity and specificity, regardless of smear result, with a 50% reduction in TTD compared to LJ. These features make MMGIT an acceptable TB diagnostic method for use in resource-limited settings.

Details

ISSN :
18157920 and 10273719
Volume :
20
Database :
OpenAIRE
Journal :
The International Journal of Tuberculosis and Lung Disease
Accession number :
edsair.doi...........2b0d538740b9c9bc47c92f2ec2cf2cf4