Effect of anti-IL17 and/or Rho-kinase inhibitor treatments on vascular remodelling in an asthma model in mice

Aims: IL17 and Rho-kinase may play a critical role in regulating inflammatory and remodeling responses in inflammatory lung diseases. Objectives: To study the effects of anti-IL17 and/or Rho-kinase inhibitor treatments on vascular changes in mice with chronic allergic inflammation. Methods: we used 64 BALB/c mice with pulmonary inflammation induced by ovalbumin that were treated with anti-IL-17A (7.5/µg/dose-ip) and/or Rho-kinase inhibitor (Y-27632-10mg/kg-intranasal) administered 1h before each ovalbumin challenge (22, 24, 26 and 28/days). Control animals were inhaled with saline. At the end of protocol, lungs were removed, morphometric analysis was performed to quantity vascular inflammatory, remodeling and oxidative stress responses. Results: OVA-Rhoi and OVA-anti-IL17 decreased all parameters (CD4+, IL5+, IL13+, IL17, Rho-Kinase2, NFκB, TIMP1, MMP9, MMP12, iNOS, FOXP3 positive cells/104μm2, collagen fibers and PGF2α contents) compared to OVA(p Conclusions: Anti-IL17 or Rho-kinase inhibitor modulate vascular inflammation, remodeling and oxidative stress responses. The association of these drugs does not potentiate the control of these responses compared to isolated treatments in this asthma model. Financial Support: FAPESP, CNPq, LIM20-HC-FMUSP.