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The application of aptamer Apt-236 targeting PvpA protein in the detection of antibodies against Mycoplasma gallisepticum
The application of aptamer Apt-236 targeting PvpA protein in the detection of antibodies against Mycoplasma gallisepticum
- Source :
- Analytical Methods. 13:3068-3076
- Publication Year :
- 2021
- Publisher :
- Royal Society of Chemistry (RSC), 2021.
-
Abstract
- Mycoplasma gallisepticum (M. gallisepticum) is the primary agent of chronic respiratory disease causing important economic losses in the poultry industry. Compared to antibodies, aptamers used to diagnose M. gallisepticum have many advantages, such as being chemically, animal-free produced and easily modifiable without affecting their affinity. Herein, a single-stranded DNA (ssDNA) aptamer Apt-236 which can specifically bind to PvpA protein of M. gallisepticum with a Kd of 1.30 ± 0.18 nM was selected successfully. An indirect blocking ELAA (ib-ELAA) for M. gallisepticum antibodies detection was also developed using Apt-236, in which M. gallisepticum antibodies would block the binding-position of aptamers. Therefor positive sera would prevent color development whereas negative sera will allow a strong color reaction. The ib-ELAA was consistent with other three widely used assays in terms of the growth and decline of the antibody response to M. gallisepticum, and showed substantial agreement with the results obtained using a commercial ELISA kit in clinical chicken sera samples. Therefore, the ib-ELAA developed in this study was a new format for aptamer application and would be an alternative method for the surveillance of M. gallisepticum.
- Subjects :
- Alternative methods
Mycoplasma gallisepticum
0303 health sciences
biology
Chemistry
General Chemical Engineering
Aptamer
010401 analytical chemistry
General Engineering
biology.organism_classification
01 natural sciences
Virology
0104 chemical sciences
Analytical Chemistry
03 medical and health sciences
Elisa kit
chemistry.chemical_compound
Antibody response
biology.protein
Antibody
DNA
030304 developmental biology
Subjects
Details
- ISSN :
- 17599679 and 17599660
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Analytical Methods
- Accession number :
- edsair.doi...........29e2e3f835e12e5a30cbab61531ab117
- Full Text :
- https://doi.org/10.1039/d1ay00515d